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. 2005 Jan;17(1):37–51. doi: 10.1105/tpc.104.026963

Figure 7.

Figure 7.

Intergenic PCR for the S3-Haplotype Using S3-RNase and S3-SFB Specific Primers in Four Possible Combinations to Determine Distance between and Transcriptional Orientation of the Genes.

The primers used on genomic DNA are S3-RNase forward with S3-haplotype–specific SFB forward (A), S3-RNase forward with S3-haplotype–specific SFB reverse (B), S3-RNase reverse with S3-haplotype–specific SFB forward (C), and S3-RNase reverse with S3-haplotype–specific SFB reverse (D). Samples are as follows: ER, Early Rivers (S1S2); Col, Colney (S5S6); EF, Emperor Francis (S3S4); Nap, Napoleon (S3S4); 2434, JI 2434 (S3′S4); S3S3′ selection; water control. Only combination (A) shows specific amplification for accessions with the S3 allele.