Figure 8.

Preliminary Restriction Maps of the S₃- and S₃′-Haplotypes of Cherry of Napoleon (S₃S₄) and JI 2434 (S₃′S₄).

Restriction enzyme positions and distances were inferred from restriction fragment size estimates on DNA gel blots (Napoleon [S₃S₄] and JI 2434 [S₃′S₄]) (Table 5), except for the regions of known sequence. Genes are represented by black boxes, with white boxes for introns. Arrows underneath gene boxes indicate the direction of transcription as deduced from the intergenic PCR (Figure 7). The breakpoint of the rearrangement in the S₃′-haplotype was deduced to be ∼3.5 kb downstream of the S₃-RNase; sequence downstream of the breakpoint (dotted line) is linked to S₃ in the progenitor. Restriction enzyme abbreviations are as follows: Ba, BamHI; E, EcoRI; K, KnpI; Nd, NdeI; Ps, PstI; Sc, ScaI; Sp, SspI; St, SstI.