Table 5.
Restriction Enzymes Used for DNA Gel Blot Analysis with S-RNase Probe and Estimated Fragment Sizes for Each of the S Alleles Present
| Estimated Size of Restriction Fragments (kb)a
| ||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Allele | BamHIb | BclI | EcoRIc | HindIII | KpnIcd | PstIce | NcoI | NdeI | PaeIc | ScaId | SstIc | SspId | XbaIc | EcoRI/HindIII |
| S1 | 14.5 | 7.5 | ∼2 | 7.5 | 21.0 | 23 | 14.5 | 6.5 | 11.5 | 5.5 | 11.5 | ∼2 | 12 | ∼2.0 |
| S3 | 9.5 | 6.0 | 13 | 8.0 | 20.0 | 7 | 8.0 | 9.5 | 7.5 | ∼4.0 | 5.0 | ∼3 + 3.5 | 22 | 7.5 |
| S3′ | 9.5 | 6.0 | 11 | 8.0 | 6.5 | 15 | 8.0 | 9.5 | 16.5 | ∼4.0 | 6.5 | ∼3 + 3.5 | 20 | 7.5 |
| S4 | >30.0 | 7.5 | ∼2 | 5.5 | 10.5 | ∼3 | 4.5 | 17.0 | 13.0 | 6.5 | 12.5 | ∼1 | 8 | ∼2.0 |
| S4′ | >30.0 | 7.5 | ∼2 | 5.5 | 10.5 | ∼3 | 4.5 | 17.0 | 13.0 | 6.5 | 12.5 | ∼1 | 8 | ∼2.0 |
a
Sizes of the bands were estimated by comparison with the 5-kb DNA ladder (Invitrogen). Please note that the size fragments under 5 kb and over 30 kb could not be estimated accurately.
b
Restriction site at 3′ end of S3-RNase (just before C5).
c
Restriction enzyme reveals a fragment size shift for S3′.
d
Restriction site in S3-RNase between C3 and RC4 (SspI), between C2 and C3 (KpnI), and just after RC4 (ScaI); the probe does not always detect both resulting fragments.
e
Restriction site at 5′ end of S3-RNase (just after C1).