Figure 2.
K+k+ RBCs were incubated with each of the indicated PUMA1 IgG isoallotype, followed by the test anti-IgG and relevant detection reagent (see methods) [dark gray histograms]. K-k+ RBCs were also incubated with each IgG isoallotype as a background control [light gray histograms]. Isoallotypes that were not recognized are indicated by (*). A separate stain was performed with each IgG isoallotype against K+,k+ and K-,k+ RBCs; the corresponding histograms for each isoallotype are overlaid in each panel.