Figure 3.

Antisense-ACLA Plants Have Complex, Aberrant Phenotypes.

(A) Representative wild-type and antisense-ACLA (αACL) plants of severe, moderate, and mild phenotypes at 80 DAI.

(B) αACL plant at 80 DAI, demonstrating the severe phenotype.

(C) to (F) Wild-type and αACL seedlings at 9 DAI germinated either in the presence ([C] to [E]) or absence (F) of kanamycin. In (C), the asterisk indicates that the wild-type seedling is chlorotic as a result of the presence of kanamycin in the media; the plant with the very severe phenotype is magnified in (D).

(G) Primary root length of wild-type and αACL seedlings at 7 DAI; n = number of plants measured.

(H) and (I) Roots of wild-type and αACL seedlings at 9 DAI.

(J) Leaves from 47-DAI wild-type plants and 85-DAI αACL plants with severe phenotype.

(K) Inflorescence stems from wild-type and αACL plants with a moderate phenotype.

(L) Shoot apical meristem from a wild-type and an αACL plant with a severe phenotype.

(M) Flowers from a wild-type plant and an αACL plant.

(N) A representative wild-type silique and siliques from αACL plants with a variety of phenotypes.

(O) Siliques from a wild-type and an αACL plant showing premature seed release.

(P) and (Q) Representative seeds from αACL plants.

(R) Representative wild-type seeds.

(S) Representative seeds from αACL plants showing a variety of phenotypes.

(T) The seed coat pigmentation of αACL seeds is lost upon sterilization with bleach.

(U) Wild-type seeds retain color during sterilization with bleach.

(V) Inflorescence stems from wild-type and αACL plants.

(W) to (AB) Micrographs of fresh vibratome sections from the base of the primary inflorescence stems of αACL plants ([W] to [Y]) and wild-type plants ([Z] to [AB]). a, anthocyanin; s, starch.