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. Author manuscript; available in PMC: 2018 Jun 1.
Published in final edited form as: Hepatology. 2017 Apr 28;65(6):2005–2018. doi: 10.1002/hep.29076

Figure 1. Expression of S1PR2 in mouse cholangiocytes and liver under cholestasis conditions.

Figure 1

(A–C): Normal mouse small cholangiocytes (MSE) and mouse large cholangiocytes (MLE) in culture were used to isolate total cellular RNA and total protein. (D). C57/BL6 wild type mice were subjected to sham operation or BDL for 3-days or two-weeks. Total RNA was isolated. (E). Mouse primary cholangiocytes were isolated from sham control or BDL mice (3-day). The mRNA levels of S1PR1, S1PR2 and S1PR3 were detected by real-time RT-PCR and normalized using GAPDH. The protein levels of S1P1, S1PR2 and S1PR3 were detected by Western Blot analysis. A and B: ***p<0.01, statistical significance relative to S1PR1, n=3; C: The representative images of the immunoblots for S1PR1-3 are shown. β-actin was used as a loading control. D and E: *p<0.05, **p<0.01, statistical significance relative to sham control, n=3–5.