Wild type and S1PR2−/− mice were subjected to 2-week BDL or sham operation as described in “Methods”. The bile acids, AST, ALT, ALP and hydroxyproline levels in serum and liver were measured using commercial kits according to the manufacturer’s instructions. (A) Total bile acids in serum. (B) Total bile acids in liver, normalized with liver weight and expressed as μM/mg. (C) AST activity in serum. (D) ALT activity in Serum. (E) ALP activity in serum. (F) Hydroxyproline in liver. Statistical significance relative to sham control, *p<0.05, **p<0.01, ***p<0.001, n=5–7; Statistical significance relative to WT BDL, *p<0.05, n=5–7.