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NRVS spectra. A, Cartoon representation of a NRVS spectrum of a 4Fe-4S cluster-containing σ-bonding ligands (e.g. OR, NHR, SR, Cl) at a 4th, unique Fe-site. B, Experimental spectra of oxidized P. furiosus D14C ferredoxin (PfFd; black), PfFd+NO (red) and [Fe4S4Cl4](Ph4P)2 (grey). C, EcIspH + 5 (red) and [13C]-5 (black).
