Identification of ACRE Genes Required for Cf-9– and Cf-4–Dependent HR.
VIGS in N. benthamiana using PVX. Seedlings were inoculated with the indicated PVX constructs to initiate silencing of the respective gene. PVX:GFP was used as a negative control.
(A) Three weeks after initiation of silencing, one leaf half was infiltrated with Agrobacteria that direct Cf-4 and Avr4 expression. Photographs of necrosis were taken 3 d after agroinfiltration.
(B) Three weeks after initiation of silencing, one leaf half was infiltrated with Agrobacteria that direct Cf-9 and Avr9 expression. Photographs of necrosis were taken 6 d after agroinfiltration.