Figure 6.

ACIK1 Specifically Regulates the Cf-9– and Cf-4–Dependent HRs.

N. benthamiana leaves were silenced as described in Figure 5A and injected with Agrobacteria expressing the following R gene/Avr gene combinations: Cf-9/Avr9, Cf-4/Avr4, Pto/AvrPto, or Rx/PVX-coat protein. In addition, N. benthamiana plants carrying the N or the Rx gene were inoculated with Agrobacteria expressing either TMV-GFP or PVX-GFP, respectively. Pictures of HR were taken 3 d after Agrobacterium infection. Accumulation of TMV-GFP and PVX-GFP was monitored by GFP fluorescence 5 DAI on inoculated leaves.