Figure 7.

LeACIK1 Is Required for Cf-9–Mediated Resistance to C. fulvum Expressing Avr9.

Transgenic Cf-9 or Cf0 tomato seedlings were inoculated with Agrobacteria expressing the indicated construct.

(A) Three weeks later, leaves were infiltrated with IF containing Avr9 (left leaf halves) or not containing Avr9 (right leaf halves). Pictures of HR symptoms were taken 1 d later under daylight (top panels) or UV light (bottom panels).

(B) Silenced tomato plants were infected with C. fulvum race 4 GUS. Leaves were stained with X-gluc 3 weeks after C. fulvum infection, and pictures were taken (top panels). In addition, fungal growth was monitored under the microscope (bottom panels).

(C) Tomato plants were silenced with the control vector (TRV:00) or the constructs indicated on the right. Total RNA was isolated from leaf discs, and LeACIK1, Cf-9, or actin transcript accumulation was analyzed by RT-PCR. Numbers of PCR cycles are indicated at the top. The control PCR with actin-specific primers was performed for every sample but is only shown for control and LeACIK1-silenced plants.