Table 2.
Full-Length and Partial ACRE cDNA Sequences Used for VIGS
| ACRE Number | 3′-RACE Product (bp) | 5′-RACE Product (bp) | Full-Length cDNA Size (bp) | GenBank Accession Number | Top Match BlastX, E-Value | Description of Top Hits |
|---|---|---|---|---|---|---|
| 1 | 1166 | AF211527 | AAR37423, 8e-52 | EREBP transcription factor | ||
| 4 | 2833 | AF211528 | AAT37497, 0.0 | N resistance protein, truncated | ||
| 11 | 986 | AY775028 | AAM63226, 6e-89 | Protein kinase, S-type receptor | ||
| 14 | 476 | AY775029 | NP_567515, 4e-32 | Triacylglycerol lipase | ||
| 19 | 387 | AY775030 | AAM61056, 5e-33 | EF-hand calcium binding protein | ||
| 20 | 417 | AY775031 | NP_194458, 2e-35 | EF-hand calcium binding protein | ||
| 31 | 972 | AF211529 | Q09011, 1e-82 | EF-hand calcium binding protein | ||
| 34 | 164 | AY775032 | No hits | No hits | ||
| 36 | 805 | AY775033 | AAL11556, 8e-78 | Chloroplast nucleoid DNA binding protein, aspartyl protease | ||
| 44 | 954(+) | AY775034 | AAO48953, 0.0 | Lipoxygenase, LoxD-like | ||
| 57 | 286 | AY775035 | BAB90781, 2e-19 | Calmodulin-related | ||
| 65 | 475 | AF211539 | AAK95311, 6e-05 | Unknown | ||
| 75 | 733 | AF211540 | AAN60336, 5e-10 | Unknown | ||
| 76 | 458 | AY775036 | NP_565634, 4e-38 | NDR1/HIN1-like | ||
| 77 | 314 | AY775037 | BI209582, 3e-05 | Protein kinase | ||
| 101 | 443 | AY775038 | O23429, 3e-19 | Synaptobrevin-related | ||
| 102 | 892 | AY775039 | AAL77654, 4e-84 | AAA-type ATPase, mitochondrial | ||
| 111B | 1000 | AF211531 | AAQ88400, 1e-92 | EREBP transcription factor | ||
| 126 | 788 | 405 | 1173 | AY220477 | NP_197017, 5e-69 | WRKY transcription factor |
| 132 | 956 | AF211532 | NP_175785, 5e-42 | RING-H2 zinc finger protein | ||
| 137 | 1171 | AF211537 | NP_915467, 3e-42 | Unknown | ||
| 140 | 518 | AY775040 | NP_196452, 9e-38 | ABA-responsive protein, GRAM domain | ||
| 141 | 1593 | 1570 | 3178 | AY220478 | CAC29254, 0.0 | Ligand-gated ion channel protein |
| 146 | 927 | AF211533 | AAO42131, 1e-31 | Unknown | ||
| 150 | 362 | AY775041 | NP_172572, 9e-13 | Protein kinase | ||
| 151 | 492 | AY775042 | JQ0956, 2e-04 | Myb transcription factor | ||
| 169 | 867 | AF211534 | AAL50092, 5e-10 | Unknown | ||
| 180 | 621 | AF211538 | NP_568661, 2e-06 | Unknown | ||
| 189 | 1245 | 850 | 1879 | AY220479 | AAG21977, e-170 | F-box/LRR protein |
| 194 | 651 | AF211535 | NP_568598, 2e-33 | Unknown | ||
| 197 | 530 | AY775043 | NP_191075, 5e-26 | Exo70 exocyst complex subunit | ||
| 216 | 749 | 654 | 1351 | AY220480 | AAK16686, e-143 | Protein kinase, NAF domain |
| 231 | 1396 | AF211536 | BAB02626, e-136 | Glycosyl transferase | ||
| 236 | 671 | AY775044 | NP_177231, 8e-40 | Protein kinase | ||
| 246 | 754 | AY775045 | NP_173960, 1e-68 | α/β Hydrolase | ||
| 256 | 949 | AY775046 | S27754, 4e-93 | Protein kinase, S-type receptor | ||
| 261 | 724 | AY775047 | NP_200709, 4e-85 | Protein kinase, APK1-like | ||
| 264 | 645 | 1172 | 1744 | AY220481 | AAL07094, e-170 | Protein kinase |
| 271 | 783 | AY775048 | AAD39930, e-101 | Protein phosphatase 2A regulatory subunit | ||
| 275 | 960(+) | 541 | 1500(+) | AY220482 | AAD13301, e-174 | Cf-type resistance protein |
| 276 | 1132 | 1489 | 2444 | AY220483 | NP_174228, 0.0 | U-box/ARM repeat protein, ARC1-like |
| 284 | 806 | 873 | 1570 | AY220484 | T09640, e-127 | Protein phosphatase 2C |