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. 2017 May 25;7:2426. doi: 10.1038/s41598-017-02587-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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TUNEL detection of apoptotic neurons in hippocampal area of SLAB51 treated and untreated AD and wt mice. Apoptotic cells are characterized by black-brownish nuclear stain, as shown in the representative images. (TUNEL (DeadEnd, Promega®) reaction, with Meyer’s hematoxylin nuclear counterstain. Bar = 200 μm.). The histogram shows the TUNEL positive cells/field. Data are representative of 5 histological section for each brain (n = 8 per sub-group). Data points marked with a hash are statistically significant compared to their respective water-treated mice (p < 0.05).