Table 2.
Kinetic parameters of PAD4 and the calcium-binding-site mutant enzymes.
| PAD4 | K m,BAEE a (mM) | k cat a (s−1) | k cat/K m,BAEE (s−1mM−1) | K 0.5,Ca (mM) | h coefficient |
|---|---|---|---|---|---|
| WT | 0.57 ± 0.33 | 14.5 ± 0.06 | 25.4 | 0.28 ± 0.05 | 1.7 ± 0.2 |
| Ca1_site mutant | 1.22 ± 0.40 | 0.02 ± 0.44 | 0.02 | 0.58 ± 0.27 | 1.0 ± 0.5 |
| Ca2_site mutant | 1.30 ± 0.15 | 1.45 ± 0.02 | 1.12 | 1.23 ± 0.44 | 1.0 ± 0.2 |
| Ca3_site mutant | 3.28 ± 0.81 | 1.84 ± 0.01 | 0.56 | 1.20 ± 0.37 | 1.0 ± 0.2 |
| Ca4_site mutant | 6.52 ± 2.40 | 0.07 ± 0.01 | 0.01 | 2.21 ± 0.38 | 1.0 ± 0.2 |
| Ca5_site mutant | 1.49 ± 0.08 | 4.54 ± 0.07 | 3.05 | 3.25 ± 0.09 | 1.8 ± 0.1 |