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. 2017 May 25;7:2400. doi: 10.1038/s41598-017-02295-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Archaeal and (b) bacterial 16S rRNA gene copy numbers (copies cm⁻³ wet sediment) and (c) BARs across a transect of eight stations in Aarhus Bay. The gray circle in (c) marks sediment samples from an organic-poor glacial sand layer at the bottom of M21. The SMTZ was reached at all stations except M21 and M22A, and varied greatly in depth and width (M24: 3.36–4.31 mbsf; M26: 2.04–3.78 mbsf; M27A: 2.62–2.90 mbsf; M29A: 2.20–2.75 mbsf; M1: 1.40–1.60 mbsf; M5: 0.47–0.58 mbsf). Free methane gas was detected at five stations (M26: ≥7.04 mbsf, M27A: ≥4.70 mbsf; M29A: ≥4.45 mbsf; M1: ≥4.00 mbsf; M5: ≥1.00 mbsf). Gravity and Rumohr cores were taken from M21, M22A, M24, M26, M27A, M29A, and M5 in May 2010, and from M1 in October 2009. Additional, increased sample-resolution Rumohr cores from M1 (M1-RL) and M5 (M5-RL) were obtained in March 2012.