Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 May 25;7:2421. doi: 10.1038/s41598-017-02653-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

T cell developmental defects in the activated CD4⁺ T cell-treated FTOC. Fetal thymic organ cultures were set up with d16 fetal thymuses (CD45.2⁺) pre-incubated without or with 2 × 10⁴ naive or activated CD4⁺ T cells (CD45.1⁺). The cultures were harvested and analysed at day 12. (a) The total number of thymocytes recovered from a single thymic lobe under different culture conditions. (b) Flow cytometric analysis of T cell development in FTOCs. Dot plots show representative profiles for CD4/CD8 staining (gated on CD45.2⁺ cells) and for CD25/CD44 staining (gated on CD45.2⁺ CD4⁻ CD8⁻ DN cells). (c,d) The percentage (c) and absolute number (d) of DN, DP, CD4 SP and CD8 SP thymocytes in naive or activated T cell-treated cultures. (e,f) The percentage (e) and absolute number (f) of different subsets of DN thymocytes in naive or activated T cell-treated cultures. DN1, CD25⁻ CD44⁺; DN2, CD25⁺ CD44⁺; DN3, CD25⁺ CD44⁻; and DN4, CD25⁻ CD44⁻. The experiments were repeated at least six times with 3- 5 thymic lobes for each group in each experiment. Data are presented as Mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001; and ns, not significant.