Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 May 25;7:2421. doi: 10.1038/s41598-017-02653-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Implication of RANK signaling in activated CD4⁺ T cell-induced effects. (a) The expression level of RANKL measured by flow cytometry in CD4 SP thymocytes, naive CD4⁺ T cells and anti-CD3/CD28-activated CD4⁺ T cells. Shallow shades represent the staining controls. (b) Fetal thymuses were co-cultured with activated T cells with or without the addition of neutralising antibodies against RANKL and analysed at day 12. Dot plots show representative profiles for CD4/CD8 staining (gated on CD45.2⁺ cells) of total thymocytes and for CD25/CD44 staining (gated on CD45.2⁺ CD4⁻ CD8⁻ DN cells) of DN cells. The number indicates the percentage of cells within the gate. (c,d) The percentage of DP and DN3 cells (c) and the absolute number of total, DP and DN3 thymocytes (d) in FTOCs under different conditions. (e,f) Flow cytrometric analysis of EpCam and Ly51 expression by MACS-enriched CD45⁻ thymic stromal cells (gated on CD45⁻ cells). Representative dot plots (e) and the ratio of mTECs versus cTECs (f) are shown. The experiments were repeated three times. Each experiment included 20–30 fetal thymic lobes for each group, which were then pooled for analysis. Each dot represents the result from a single experiment. Data are presented as Mean ± SEM. *p < 0.05, **p < 0.01; ***p < 0.001.