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. 2017 May 26;10:157. doi: 10.3389/fnmol.2017.00157

Figure 1.

Figure 1

Onecut (OC) factors are differentially and dynamically distributed in dI2 to dI4 populations. (A–F) Immunodetection of HNF-6, OC-2 or OC-3 (red) in Foxd3+ (blue) Brn3a+ (green) dI2 neurons on transverse sections of thoracic spinal cord at e10.5 (A–C) and e12.5 (D–F). HNF-6 (A–A”), OC-2 (B–B”) and OC-3 (C–C”) are visible in a few dI2 cells at e10.5 whereas they are not detected at e12.5 (D–F”). The number (G) and percentage (H) of OC-positive dI2 neurons per hemisection were quantified accordingly. (I–N) Immunodetection of HNF-6, OC-2 or OC-3 (red) in Isl1+ (blue) Brn3a+/Arx+ (green) dI3 neurons on transverse sections of thoracic spinal cord at e10.5 (I–K) and e12.5 (L–N) demonstrates that OC factors are present in dI3 from e10.5 to e12.5 with a slight predominance of HNF-6 (I,L) compared to OC-2 (J,M) or OC-3 (K,N). Despite an increase in the number of OC+ dI3 neurons from e10.5 to e12.5 (O), the percentage of dI3 neurons containing OC factors (P) is reduced at embryonic stage e12.5. (Q–V) Immunodetection of HNF-6, OC-2 or OC-3 (red) in Lbx1+ (blue) Lhx1/5+ (green) dI4 neurons on transverse sections of thoracic spinal cord at e10.5 (Q–S) and e12.5 (T–V) shows that OC factors are present in early dI4 neurons, HNF-6 (Q–Q”) being the most represented. At e12.5, HNF-6 (T–T”) and OC-2 (U–U”) are still detected in a few dI4 neurons while OC3 (V–V”) is barely detectable. Quantifications at embryonic stages e10.5 and e12.5 show that number (W) and percentage (X) of OC-positive dI4 neurons decrease from e10.5 to e12.5. The pictures show left hemisections as indicated to the left. Dashed lines delineate the spinal cord and the lumen of the ventricle. Insets are magnified views of boxed regions. Arrowheads point to double-labeled cells. Mean values ±SEM, n ≥ 3. Scale bars = 100 μm.