Figure 3.

Mitochondrial sirtuin levels are highest in primary cultured neurons compared to microglia, astrocytes and oligodendrocytes. mRNA levels of Sirt3 (A), Sirt4 (B) and Sirt5 (C) were determined in primary cultured microglia, neurons, oligodendrocytes and astrocytes of C57BL/6J mice using qPCR. Data was normalized to TATA box binding protein (Tbp) and RNA polymerase 2 (Polr2a). Protein levels of SIRT3 were determined by western blot (D). Protein data was normalized to the total protein amount determined by MemCode staining. Data were analyzed by the one-way ANOVA followed by Tukey’s multiple comparison test, n = 5–8. Data are shown as mean ± SEM. ns = P > 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001.