Figure 4.

Lipopolysaccharide (LPS) stimulation decreases Sirt3 mRNA levels in primary cultured microglia but not in astrocytes. Cultured primary microglia (A,C,E,G) and astrocytes (B,D,F,H) were treated with 1 μg/ml LPS for 0, 2, 4, 8, 12 and 24 h. Sirt3 (A, B), Sirt4 (C,D) and Sirt5 (E,F) mRNA levels were determined by qPCR and normalized to TATA box binding protein (Tbp), RNA polymerase 2 (Polr2a) for microglia and additionally to β-actin (Actb) for astrocytes. SIRT3 protein levels were determined by western blot and normalized to the total protein amount determined by MemCode staining (G,H). Data were normalized to the untreated control and analyzed by the nonparametric Kruskal-Wallis test, followed by Dunn’s multiple comparison test with n = 2–8 for microglia and n = 3–4 for astorcytes. Data are shown as mean ± SEM. ns = P > 0.05, *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001.