Figure 5.

Sirt3 levels show a decreasing tendency in the spinal cord and cortex of peroxisome proliferative-activated receptor gamma coactivator 1 alpha (PGC-1α) KO mice. Sirt3 (A,B) mRNA levels were determined by qPCR in the spinal cord (A) and cortex (B) of 90 day old B6.129-Ppargc1a^tm1Brsp/J (PGC-1α KO) mice and respective controls. mRNA levels were normalized to TATA box binding protein (Tbp), RNA polymerase 2 (Polr2a) and β-actin (Actb). Levels are shown in percent of the wt control. Protein levels of SIRT3 were determined in the spinal cord (C) and cotex (D) by western blot and data were normalized to total protein amount determined by MemCode staining. The nonparametric Mann-Whitney test was used for analysis with n = 3. Data are shown as mean ± SEM.