Table 2.
T9SS cargo proteins.
| Porphyromonas gingivalisa | |||||
|---|---|---|---|---|---|
| Locus Tag | |||||
| W83 NC_002950.2 | ATCC33277 NC_010729.1 | Protein accession number | Protein description | References | |
| PG_RS00120 | PG0026 | PGN_0022 | WP_005874469.1 | PorU; surface C-terminal sortase | Glew et al., 2012; Veith et al., 2013; Gorasia et al., 2015 |
| PG_RS00835 | PG0182 | PGN_0291 | WP_010955943.1 | Mfa5; VWA domain-containing protein [von Willebrand factor (vWF) type A domain] | Hasegawa et al., 2016 |
| PG_RS00840 | PG0183 | no PGN | WP_043876389.1 | Hypothetical protein containing VWA domain identical to that in PG0182 (circa 430 residues); lipoprotein | Found only by proteomic analysisa |
| PG_RS01060 | PG0232 | PGN_0335 | WP_005873522.1 | CPG70; zinc carboxypeptidase | Veith et al., 2004; Shoji et al., 2011; Zhou et al., 2013 |
| PG_RS01560 | PG0350 | PGN_1611 | WP_005873799.1 | Internalin; hypothetical protein; leucine-rich repeats (x8) | Found only by proteomic analysisa |
| PG_RS01820 | PG0410 | no PGN | WP_005873803.1 | Hypothetical gingipain-like peptidase C25 | |
| PG_RS01825 | PG0411 | PGN_1556 | WP_010956006.1 | T9SS C-terminal target domain-containing protein | Found only by proteomic analysisa |
| PG_RS02195 | PG0495 | PGN_1476 | WP_010956042.1 | T9SS C-terminal target domain-containing protein | Found only by proteomic analysisa |
| PG_RS02240 | PG0506 | PGN_1466 | WP_010956050.1 | RgpB; arginine specific gingipain B, cysteine protease | Pike et al., 1994; Seers et al., 2006; Guo et al., 2010; de Diego et al., 2016 |
| PG_RS02455 | PG0553 | PGN_1416 | WP_010956068.1 | PepK; lysine specific serine endopeptidase | Sato et al., 2013; Nonaka et al., 2014; Veith et al., 2014 |
| PG_RS02700 | PG0611 | PGN_0654 | WP_043876409.1 | Hypothetical protein | Found only by proteomic analysisa |
| PG_RS02710 | PG0614 | PGN_0657 | WP_005874506.1 | Hypothetical protein | Found only by proteomic analysisa |
| PG_RS02720 | PG0616 | PGN_0659 | WP_005874521.1 | HBP35 (hemin binding protein 35) | Shoji et al., 2010, 2011 |
| PG_RS02765 | PG0626 | no PGN | WP_005874512.1 | T9SS C-terminal target domain-containing protein | Found only by proteomic analysisa |
| PG_RS02890 | PG0654 | PGN_0693 | WP_005873571.1 | T9SS C-terminal target domain-containing protein | Found only by proteomic analysisa; Glew et al., 2012 |
| PG_RS03370 | PG0769 | PGN_0795 | WP_010956121.1 | Fibronectin; hypothetical proteinb | Found only by proteomic analysisa; Sato et al., 2013 |
| PG_RS03450 | PG0787 | PGN_0810 | WP_005873930.1 | T9SS C-terminal target domain-containing proteinc | Found only by proteomic analysisa |
| PG_RS04535 | PG1030 | PGN_1321 | WP_005874101.1 | T9SS C-terminal target domain-containing protein | Found only by proteomic analysisa |
| PG_RS05835 | PG1326 | PGN_1115 | WP_005875446.1 | Hemagglutinin | Found only by proteomic analysisa |
| PG_RS06055 | PG1374 | PGN_0852 | WP_005874331.1 | T9SS C-terminal target domain-containing protein, leucine-rich repeats (x7) | Found only by proteomic analysisa; Glew et al., 2012 |
| PG_RS06255 | PG1424 | PGN_0898 | WP_005873463.1 | PPAD; peptidylarginine deiminase | Sato et al., 2013; Koziel et al., 2014; Goulas et al., 2015 |
| PG_RS06260 | PG1427 | PGN_0900 | WP_005873781.1 | Periodontain; peptidase C10; PrtT-related | Nelson et al., 1999 |
| PG_RS06835 | PG1548 | PGN_0561 | WP_043876505.1 | PrtT; cystein protease (domain peptidase C10) | Madden et al., 1995; Gorasia et al., 2015 |
| PG_RS07070 | PG1604 | PGN_0509 | WP_010956350.1 | PorZ; surface B-propeller protein | Lasica et al., 2016 |
| PG_RS07920 | PG1795 | PGN_1770 | WP_005874140.1 | Hypothetical protein | Found only by proteomic analysisa |
| PG_RS07930 | PG1798 | PGN_1767 | WP_005874135.1 | T9SS C-terminal target domain-containing protein | Found only by proteomic analysisa |
| PG_RS08090 | PG1837 | PGN_1733 | WP_043876452.1 | HagA (hemagglutinin A, 8 HA domains) | Shi et al., 1999; Glew et al., 2012; Saiki and Konishi, 2014 |
| PG_RS08105 | PG1844 | PGN_1728 | WP_043876454.1 | Kgp; lysine specific gingipain, cysteine protease | Pike et al., 1994; Veith et al., 2002 |
| PG_RS08700 | PG1969d | no PGN | WP_010956456.1 | T9SS C-terminal target domain-containing protein | Found only by proteomic analysisa |
| PG_RS08940 | PG2024 | PGN_1970 | WP_010956476.1 | RgpA; arginine specific gingipain A; cysteine protease | Pike et al., 1994; Veith et al., 2002; Glew et al., 2012 |
| PG_RS09310 | PG2100 | no PGN | WP_005873768.1 | T9SS C-terminal target domain-containing protein; TapC | Kondo et al., 2010; Sato et al., 2013 |
| PG_RS09320 | PG2102 | PGN_0152 | WP_005873754.1 | T9SS C-terminal target domain-containing protein; TapA | Kondo et al., 2010; Glew et al., 2012; Sato et al., 2013 |
| PG_RS09640 | PG2172 | PGN_0123 | WP_005874973.1 | Hypothetical protein | Found only by proteomic analysisa; Glew et al., 2012 |
| PG_RS09755 | PG2198 | PGN_2065 | WP_005874281.1 | Hypothetical protein; peptidase | Found only by proteomic analysisa |
| PG_RS09850 | PG2216 | PGN_2080 | WP_010956525.1 | Hypothetical protein | Found only by proteomic analysisa; Glew et al., 2012 |
| Tannerella forsythia ATCC43037 | |||||
| Tanf_03370 | WP_046824918.1 | TfsA (surface layer protein A), classical CTD | Tomek et al., 2014 | ||
| Tanf_03375 | WP_046824919.1 | TfsB (surface layer protein B), classical CTD | Tomek et al., 2014 | ||
| Tanf_04820 | WP_046825062.1 | BspA, cell surface antigen, leucine rich protein, classical CTD | Veith et al., 2009; Friedrich et al., 2015 | ||
| Tanf_06225 | WP_046825275.1 | Forsilysin, metalloprotease, KLIKK-type CTD | Narita et al., 2014 | ||
| Tanf_00450 | WP_070098098.1 | Mirolysin, metalloprotease, KLIKK-type CTD | Karim et al., 2010; Ksiazek et al., 2015a,b; Koneru et al., 2017 | ||
| Tanf_06550 | D0EM77.2 | Karilysin, metalloprotease, KLIKK-type CTD | Karim et al., 2010; Narita et al., 2014; Ksiazek et al., 2015a; Koneru et al., 2017 | ||
| Tanf_00440 | AIZ49398.1 | Mirolase, serine protease, KLIKK-type CTD | Karim et al., 2010; Ksiazek et al., 2015a,b; Koneru et al., 2017 | ||
| Tanf_09450, Tanf_06530 (not merged in one contig) | AKG97061.1 | Miropsin-1, serine protease, KLIKK-type CTD | Ksiazek et al., 2015b | ||
| Tanf_06530 | WP_046825306.1 | Miropsin-2, serine protease KLIKK-type CTD | Narita et al., 2014 | ||
| F. johnsoniae UW101e | |||||
| Fjoh_4555 | WP_012026520.1 | ChiA, chitinase | Rhodes et al., 2010; Kharade and McBride, 2014 | ||
| Fjoh_0979 | WP_012023065.1 | SprB, surface adhesin, necessary for gliding motility | Rhodes et al., 2010; Shrivastava et al., 2013 | ||
| Fjoh_0808 | WP_052295174.1 | RemA, mobile surface adhesin, necessary for gliding motility | Shrivastava et al., 2012, 2013 | ||
a
All P. gingivalis cargo proteins excluding PG0410 (no PGN) and PG1548 (PGN_0561) were originally found by Veith et al. (2013).
b
PG0769 (PGN_0795) processing is unclear. Protein is devoid of N-terminal cleavage signal for periplasm transport (searched with SignalP and LipoP servers) as well as T9SS CTD domain.
c
PG0787 (PGN_0810) is a very small peptide (80 aa) devoid of N-terminal cleavage signal for periplasm transport (searched in SignalP and LipoP servers), however its last 66 aa constitute a classical T9SS CTD domain.
d
PG1969 processing is unclear. Protein is devoid of N-terminal cleavage signal for periplasm transport (searched with SignalP and LipoP servers) but contains T9SS CTD domain.
e
Proteins listed are the best studied among other identified T9SS cargos of F. johnsoniae. For more information please see Kharade and McBride (2015).