Fig. 3.

Effect of TLR-2/-4 antagonists on LTA-induced synthesis of IL-8. A549 cells were either sham-incubated (control) or exposed to 1 µg/ml of LTA in the absence or presence of neutralizing antibodies targeting TLR-2 or TLR-4 for 48 h. Release of IL-8 into the cell supernatants was analyzed by ELISA technique and is given as ΔIL-8 (pg/ml), which was calculated by subtracting IL-8 values of sham-incubated cells from LTA-stimulated cells. Data are expressed as means ± SEM of at least six independent experiments. Values marked with an asterisk differ significantly from controls (p < 0.05)