Table 2.
Primers used for PCR
| Amplified locus | Primer sequences (5'-3') | Annealing temperature | Reference |
|---|---|---|---|
| Ixodes sp. ITS2 | F-ITS2 (cacactgagcacttactctttg) R1-ITS2 (actggatggctccagtattc) |
57 °C | [58] |
| I. persulcatus cox1 gene | Ixodes-F (acctgatatagctttccctcg) Ipers-R (ttgattcctgttggaacagc) |
55 °C | [69] |
| I. pavlovskyi cox1 gene | Ixodes-F (acctgatatagctttccctcg) Ipav-R (taatccccgtggggacg) |
55 °C | [69] |
| TBEV E-NS1 genes | E7 (ggcatagaaaggctgacagtg) E10 (gatacctctctccacacaaccag) |
52 °C | [34] |
| E9 (acagtgataggagaacacgcctggg) E8 (cagccaggaggaagctcatggac) |
52 °C | [34] | |
| KEMV segment 1 | Kem1s_1 (attcaaattacgacacgcacatgac) Kem1s_2 (gtatcgtcgccgacgtacatctc) |
56 °C | [70] |
| Kem1s_3 (gctcatcgaagcgggatacgg) Kem1s_4 (gcgtagagttctctcccgacagatg) |
56 °C | [70] | |
|
Borrelia burgdorferi (s.l.) 5S-23S rRNA intergenic spacer |
NC1 (cctgttatcattccgaacacag) NC2 (tactccattcggtaatcttggg) |
50 °C | [14] |
| NC3 (tactgcgagttcgcgggag) NC4 (cctaggcattcaccatagac) |
54 °C | [71], modified | |
|
B. miyamotoi
glpQ gene |
Q1 (caccattgatcatagctcacag) Q4 (ctgttggtgcttcattccagtc) |
50 °C | [44] |
| Q3 (gctagtgggtatcttccagaac) Q2 (cttgttgtttatgccagaagggt) |
54 °C | [44] | |
|
B. burgdorferi (s.l.) p83/100 gene |
F7 (ttcaaagggatactgttagagag) F10 (aagaaggcttatctaatggtgatg) |
50 °C | This study |
| F5 (acctggtgatgtaagttctcc) F12 (ctaacctcattgttgttagactt) |
54 °C | This study | |
|
B. burgdorferi (s.l.) clpA gene |
clpAF1237 (aaagatagatttcttccagac) clpAR2218 (gaatttcatctattaaaagctttc) |
55 → 48 °C | [72] |
| clpAF1255 (gacaaagcttttgatattttag) clpAR2104 (caaaaaaaacatcaaattttctatctc) |
50 °C | [72] | |
|
Anaplasmataceae
16S rRNA gene |
Ehr1 (gaacgaacgctggcggcaagc) Ehr2 (agtaycgraccagatagccgc) |
57 °C | [45] |
| Ehr3 (tgcataggaatctacctagtag) Ehr4 (ctaggaattccgctatcctct) |
60 °C | [45] | |
|
A. phagocytophilum
16S rRNA gene |
HGE1 (cggattattctttatagcttgc) HGE2 (cttaccgaaccgcctacatg) |
55 °C | [45] |
|
E. muris
16S rRNA gene |
Em1 (cgaacggatagctacccatagc) Em2 (cgctccaaagttaagctttggt) |
55 °C | [45] |
|
Anaplasmataceae
groESL operon |
HS1-f (cgycagtgggctggtaatgaa) HS6-r (ccwccwggtacwacaccttc) |
55 °C | [73], modified |
| HS3-f (atagtyatgaaggagagtgat) HSVR (tcaacagcagctctagtwg) |
50 °C | [74] | |
|
Rickettsia spp. gltA gene |
glt1 (gattgctttacttacgaccc) glt2 (tgcatttctttccattgtgc) |
52 °C | [49] |
| glt3 (tatagacggtgataaaggaatc) glt4 (cagaactaccgatttctttaagc) |
53 °C | [49] | |
| “Ca. R. tarasevichiae” gltA gene | RT1 (tactaaaaaagtcgctgttcattc) RT2 (tgttgcaaacatcatgcgtaag) |
56 °C | [49] |
| SFGR gltA | RH1 (gtcagtctactatcacctatatag) RH3 (taaaatattcatctttaagagcga) |
54 °C | [49] This study |
|
Babesia spp. 18S rRNA gene |
BS1 (gacggtagggtattggcct) BS2 (attcaccggatcactcgatc) |
58 °C | [46] |
| BS3 (taccggggcgacgacgggtg) BS5 (cgaggcagcaacgggtaacg) BS4 (agggacgtagtcggcacgag) |
62 °C | [46] |