Table 2.
Statistics of trimmed reads and unigenes of L.glauca fruits by different sequencing strategies
| Sequencing strategy and assembly | Trimmed reads | Unigenes | ||
|---|---|---|---|---|
| Number | Mean length (bp) | Number | Mean length (bp) | |
| Illumina sequencing | ||||
| 50 DAF | 27,921,143 | 92.85 | 130,827 | 608.44 |
| 125 DAF | 29,036,556 | 92.63 | ||
| 150 DAF | 24,297,910 | 92.12 | ||
| 454 sequencing | 957,341 | 518.48 | 70,432 | 822.74 |
| Short- and long-read assembly | 60,031 | 1061.95 | ||
Three representative fruit samples from 50, 125 and 150 DAF and one mixed sample from seven different developing stages were selected for Illumina and 454 sequencing, respectively. The unigenes separately obtained from long read and short read (201,259 in total) was reconciled by TGICL software