Fig. 1.

Disulfide and all-thiol HMGB1 dose response effects on abdominal mechanical thresholds. (a) vehicle control, (b) 1 μg (n = 4) and (c) 2 μg (n = 3) disulfide HMGB1 did not affect abdominal mechanical threshold. (d) 5 μg (n = 5) disulfide HMGB1 significantly increased abdominal sensitivity using a 0.07 g filament. (e) 10 μg (n = 6) and (f) 20 μg (n = 3) disulfide HMGB1 significantly induced abdominal hypersensitivity using all four von Frey filaments. None of the all-thiol HMGB1 doses (g) 1 μg (n = 3), (h) 2 μg (n = 3), (i) 5 μg (n = 4), (j) 10 μg (n = 5) and (k) 20 μg (n = 3) changed abdominal mechanical sensitivity. *p < 0.05, **p < 0.01 compared with pre-instillation