Fig. 3.

TLR4 or RAGE antagonist pretreatment prevented abdominal mechanical hypersensitivity induced disulfide HMGB1. (a) 10 μg disulfide HMGB1 significantly increased abdominal mechanical sensitivity (percent responses) using all four von Frey filaments (n = 6). (b) Intravesical TLR4 antagonist TAK242 blocked disulfide HMGB1 induced abdominal hypersensitivity (n = 4). (c) Intraperitoneal TAK242 reduced mechanical hypersensitivity induced by 10 μg disulfide HMGB1 (n = 5). (d) Intravesical infusion of RAGE antagonist PSF-ZM1, however, did not affect abdominal mechanical hypersensitivity induced by disulfide HMGB1 (n = 6). (e) Intraperitoneal injection of PSF-ZM1 prevented disulfide HMGB1 induced mechanical hypersensitivity (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001 Twenty-four hours post-instillation compared with pre-instillation