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. 2017 May 25;15:105. doi: 10.1186/s12957-017-1172-x

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — PTK7 negatively regulates cell apoptosis in esophageal squamous tumor cells. Apoptosis of the PTK7 knockdown cells and the control cells was evaluated by flow cytometry after double staining of Annexin V-FITC-propidium iodide for TE-5 (a) and TE-9 (b) cells. Apoptosis of PTK7-overexpressing and control cells was measured by flow cytometry after double staining of Annexin V-FITC and propidium iodidefor TE-5 (c) and TE-9 (d) cells. e Quantitative real-time PCR was performed for major apoptosis regulators, and the relative mRNA levels are presented for siPTK7 vs. control cells. (*p < 0.05, **p < 0.01, Student’s t test)