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. 2017 May 25;15:105. doi: 10.1186/s12957-017-1172-x

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — PTK7 positively cell invasion in esophageal squamous tumor cells. a and b western blots of PTK7 and E-cadherin were performed for the siPTK7 and control cells in TE-5 (a, left panel) and TE-9 (b, left panel). Transwell assays were performed in TE-5 and TE-9 (a and b, middle panels). Quantification of the results shown in (a and b, right panels). c and d PTK7 was overexpressed in TE-5 and TE-9 cells. Western blots of PTK7 and E-cadherin (left panels), Transwell migration assays (middle panels), and the quantification of cell migration (right panels) of the PTK7-overexpressing and control cells are presented. (mean ± S.D., n = 3, *p < 0.05, **p < 0.01, Student’s t test)