. 2017 May 25;9:30. doi: 10.1186/s13099-017-0179-8

Table 2.

Antibody panels used for immunophenotyping by flow cytometry

Cell population addressed	Antigen	Clone	Isotype	Fluorochrome	Labelling strategy	Source of primary Ab
Myeloid cells, B cells and CD4⁺ T cells	CD3	PPT3	IgG1	BV 421^a	Biotin–streptavidin	In-house
Myeloid cells, B cells and CD4⁺ T cells	CD79α	HM57	IgG1	PE	Direct conjugation	Dako
	CD172α	74-22-15	IgG1	Alexa Fluor 647^b	Direct conjugation	In-house
	CD4	74-12-4	IgG2b	Alexa 488^c	Secondary antibody	In-house
T cells and NK-cell subpopulations	CD3	PPT3	IgG1	BV 421^a	Biotin–streptavidin	In-house
	CD4	74-12-4	IgG2b	Alexa Fluor 488^c	Secondary antibody	In-house
	CD8α	11/295/33	IgG2a	PE^d	Secondary antibody	In-house
	NKp46	VIV-KM1	IgG1	Alexa Fluor 647^b	Direct conjugation	In-house
γδ T-cell subpopulations	TCR-γδ	PPT16	IgG2b	BV421^a	Biotin–streptavidin	In-house
	CD8α	11/295/33	IgG2a	Alexa Fluor 647^b	Direct conjugation	In-house
	CD27	b30c7	IgG1	PE^e	Secondary antibody	In-house
	CD2	MSA4	IgG2a	Alexa Fluor 488^f	Direct conjugation	In-house

^aBrilliant Violet 421 streptavidin, BioLegend, San Diego, CA, USA

^bAlexa Fluor 647 Protein Labelling Kit, Invitrogen, Carlsbad, CA, USA

^cGoat anti-Mouse IgG2b-Alexa Fluor 488, Invitrogen, Carlsbad, CA, USA

^dGoat anti-Mouse IgG2a-PE, Southern Biotech, Birmingham, AL, USA

^eGoat anti-Mouse IgG1-PE, Southern Biotech, Birmingham, AL, USA

^fAlexa Fluor 488 Protein Labelling Kit, Invitrogen, Carlsbad, CA, USA