Fig. 1.

quantGenius workflow. The data for the target and reference genes is imported and quality controlled. Relative copy numbers are calculated using standard curve parameters and normalized. The final results are exported as a gene-sample matrix. The calculation steps are marked with bold, while the calculated parameters are listed below with regular letters. All the formulas used for calculations (Equations 1–25) are available in the Additional file 2. Abbreviations: Cq – quantification cycle, Rel. - relative no.- number, Std. – standard, QC – quality control, CV - coefficient of variation, CqExtC – Cq value of the extraction control, CqLOQ – Cq value of the limit of quantification