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. 2017 May 25;18:276. doi: 10.1186/s12859-017-1688-7

Fig. 2.

Fig. 2

Screenshot of the calculation of the reference copy numbers. An example of two reference genes (COX and EF-1) is shown. The second imported reference gene (EF-1) is scaled to the average of the first reference gene imported and the average of both values is calculated. The calculations are performed for each dilution separately