Fig. 4.

quantGenius quality control-based decision support system (DSS). Decision tree case of (a) simple (one-dilution) calculation and (b) two-dilution calculation. The following QC control steps are implemented hierarchically: 1) extraction control, 2) limit of detection 3) limit of quantification, and 4) individual sample efficiency of amplification control. Based on the DSS, the final result is calculated (blue boxes), modified (orange boxes) or not given (red boxes) and warnings are issued. Abbreviations: Cq – quantification cycle, CqExtC – Cq value of the extraction control, no. – number, CqLOQ – Cq value of the limit of quantification, dil. – dilution, QC – quality control, CV - coefficient of variation