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. 2017 May 5;3(5):501–509. doi: 10.1021/acscentsci.7b00134

Figure 4.

Figure 4

Experimental validation of BA–protein interactions by immunoblotting. Each protein is transiently overexpressed in HeLa cells with a His tag, and the cells are labeled with P2. The labeled proteins are enriched and then eluted for immunoblotting with an anti-His antibody (“Elution”). The samples without UV cross-linking or with 2× BA competition are used as control. Equal loading is validated by immunoblotting of input samples before enrichment (“Input”). Top panel: NTCP (a BA transporter), SLC25A6, and PLD3 are known BA–protein interactions that have been reported before. Bottom panel: CPT1A, ADPGK, and COMT are novel BA–protein interactions discovered in this study. The normalized intensities are shown below each band.