Fig. 3.

The effect of PAX6 and FOXC1 overexpression in hESCs. a Schematic representation of time course of mRNA transfection of PAX6 and FOXC1. b Phase-contrast images of hESCs transfected with PAX6 and FOXC1 mRNAs. Scale bar 100 µm. c A phase-contrast image of PAX6 and FOXC1 transfected hESCs at Day 5 in basal medium. Scale bar 100 µm. d Relative mRNA expression levels of representative ectodermal markers (FGF5, LEFTY2), branching morphogenesis marker (BARX2), and lacrimal gland epithelial markers (KRT15, AQP5, and LTF) after transfecting PAX6 and FOXC1 mRNAs into hESCs. Quantitative PCR analyses of total RNA extracts on day 5, or the control cells also grown for the same length of time (5 days) as the experimental cells. Error bars represent mean ± SD of three samples. e Relative mRNA expression profiles of the developmental markers including lacrimal gland epithelial markers after PAX6 mRNA induction in hESCs on Day 5, or the control cells also grown for the same length of time (5 days) as the experimental cells. Error bars represent mean ± SD of three samples