Table 2.
Morphological changes of cells transfected with each transcription factor and their combinations in hESCs
| Combination of TF | Morphological change (Day 5) | mRNA expression of PAX6, BARX2, KRT15, AQP5, and LTF (Day 5) |
|---|---|---|
| None | Spindle | No |
| PAX6 | Spindle | No |
| FOXC1 | Smooth | No |
| SIX1 | Smooth | No |
| SIX2 | Round | BARX2a |
| PAX6 + FOXC1 | Elongate | BARX2, KRT15, AQP5 |
| PAX6 + SIX1 | Smooth/spindle | No |
| PAX6 + SIX2 | Round/spindle | No |
| FOXC1 + SIX1 | Spindle | No |
| FOXC1 + SIX2 | Elongate | BARX2, AQP5a |
| PAX6 + FOXC1 + SIX1 | Elongate | PAX6, BARX2, KRT15, AQP5, LTF |
| PAX6 + FOXC1 + SIX2 | Spindle/elongate | BARX2a |
aThe details of partial expression of lacrimal gland-related markers are shown in Supplementary information