Analytical sensitivity of the lateral flow dipstick test in detecting antigen from lysed E. histolytica trophozoites spiked in a stool sample of healthy individual.
Notes: Arrow indicates the position of the visible dot. Lane 1: dipstick tested with non-spiked stool; Lanes 2–6: dipsticks tested with stool spiked with 10, 100, 1,000, 10,000 and 100,000 cells ml−1 of trophozoites, respectively. The limit of detection of the dipstick was determined to be 1,000 cells ml−1.