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The effect of NAM supplementation on intracellular NAD content. HepG2 cells were treated with NAM (1 and 5 mM) for 2 and 8 hours, respectively. Intracellular NAD+ and NADH levels were determined. A. NAM increases cellular NAD+ concentration. B. NAM increases cellular NAD+/NADH ratio. * p < 0.05 vs. control. All values are denoted as means ± SD from three or more independent studies.
