Table 2. ACSL enzyme activity in pure beta cells is concentrated in insulin secretory granules and mitochondria.
ACSL enzyme activity was measured with palmitate as a substrate as described under Material and Methods. The specific enzyme activity of the homogenate of whole cells is defined as 1.
| Cellular Fraction | Relative Specific ACSL Activity/mg Protein |
|---|---|
| Homogenate of whole cells | 1 |
| Insulin Secretory Granules | 4.1 |
| Mitochondria | 1.9 |