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. Author manuscript; available in PMC: 2018 Apr 1.
Published in final edited form as: Mol Cancer Ther. 2017 Jan 30;16(4):763–772. doi: 10.1158/1535-7163.MCT-16-0644

Figure 2.

Figure 2

NP shows antioxidant activity and NOX4 reduction in three TNBC cell lines (BT549, MDA-MB-231, and LM2-4luc+/H2N). (A) ROS levels of the cells after 24 h treatment with siSCR-NP (50 nM), DPI (5 µM), or NAC (20 mM), followed by 1 h treatment with 100 µM of menadione, assessed by CellROX flow cytometry. (B) Expression levels of NOX4 mRNA at 24 h and (C) NOX4 protein at 72 h of the three cell lines after receiving similar treatments with (A) for 24 h but without menadione. All mRNA data were measured by qPCR and normalized to GAPDH expression. Data are presented as mean ± SD from 3 independent experiments. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001 vs. untreat.