Table 2.
Summary of the QTL for saponin contents detected in PT-RILs
| Simple Interval Mapping method | Marker Interval flanking QTL peak (marker position cM) | Chromosome (linkage group) | Highest LOD | Marker with highest LOD (marker position cM) | Alleles with increasing effect | Additive effect | Explained phenotypic variance (%) | ||
|---|---|---|---|---|---|---|---|---|---|
| Hypocotyl | Group A saponin | Tsukuba | Not detected | ||||||
| Nagano | Sat_344 ( 26.8)–Sat_356 (47.6) | 5 (A1) | 5.0 | Sat_384 (36.1) | Peking | 0.24 | 21.9 | ||
| Kyoto | Not detected | ||||||||
|
|
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| Group B saponin | Tsukuba | Satt276 (24.0)– Sat_356 (47.6) | 5 (A1) | 4.9 | Sat_384 (36.1) | Peking | 0.14 | 22.0 | |
| Nagano | Sat_137 (0.0)– Sat_356 (47.6) | 5 (A1) | 11.8 | Satt364 (35.0) | Peking | 0.20 | 44.2 | ||
| Kyoto | Satt276 (24.0)– Sat_356 (47.6) | 5 (A1) | 4.2 | Satt526 (34.4) | Peking | 0.12 | 18.8 | ||
|
| |||||||||
| Cotyledon | Group A saponin | Tsukuba | Sat_213 (77.5)– Satt371 (132.5) | 6 (C2) | 6.4 | T (92.5) | Tamahomare | 0.03 | 27.7 |
| Nagano | Sat_213 (77.5)– Satt316 (103.1) | 6 (C2) | 5.1 | Sat_312 (94.1) | Tamahomare | 0.02 | 22.3 | ||
| Kyoto | Satt302 (81.4)– Sat_218 (98.3) | 12 (H) | 3.0 | Sat_175 (91.8) | Peking | 0.05 | 13.8 | ||
| Kyoto | Sat_213 (77.5)– Satt316 (103.1) | 6 (C2) | 3.4 | Sat_312 (94.1) | Tamahomare | 0.05 | 15.5 | ||
|
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| Group B saponin | Tsukuba | Not detected | |||||||
| Nagano | Satt590 (0.0)– Satt567 (20.9) | 7 (M) | 3.4 | Satt150 (3.7) | Peking | 0.02 | 15.5 | ||
| Kyoto | Not detected | ||||||||
| Composite Interval Mapping method | Marker Interval flanking QTL peak (marker position cM) | Chr. (linkage group) | Highest LOD | Marker with highest LOD (marker position cM) | Alleles with increasing effect | Additive effect | Explained phenotypic variance (%) | ||
|---|---|---|---|---|---|---|---|---|---|
| Hypocotyl | Group A saponin | Tsukuba | Sat_344 (26.8)–Sat_356 (47.6) | 5(A1) | 3.1 | Sat_384 (36.1) | Peking | 0.17 | 9.2 |
| Tsukuba | Sct_067 (15.6)–Satt424 (56.5) | 8 (A2) | 3.7 | I (49.2) | Peking | 0.07 | 17.0 | ||
| Nagano | Sat_344 (26.8)–Sat_356 (47.6) | 5 (A1) | 4.7 | Sat_384 (36.1) | Peking | 0.24 | 14.6 | ||
| Kyoto | Not detected | ||||||||
|
|
|||||||||
| Group B saponin | Tsukuba | Sat_344 (26.8)–Sat_356 (47.6) | 5 (A1) | 6.9 | Sat_384 (36.1) | Peking | 0.14 | 24.8 | |
| Tsukuba | Sat_092 (37.1)–Satt397 (52.9) | 17 (D2) | 4.2 | Satt669 (50.3) | Tamahomare | 0.07 | 11.2 | ||
| Nagano | Sat_344 (26.8)–Sat_356 (47.6) | 5 (A1) | 14.1 | Sat_371 (33.8) | Peking | 0.20 | 39.9 | ||
| Kyoto | Sat_344 (26.8)–Sat_356 (47.6) | 5 (A1) | 4.0 | Sat_371 (33.8) | Peking | 0.12 | 23.7 | ||
|
| |||||||||
| Cotyledon | Group A saponin | Tsukuba | Satt277 (90.3)–Satt460 (95.7) | 6 (C2) | 5.7 | Satt134 (93.6) | Tamahomare | 0.03 | 22.0 |
| Nagano | Sat_213 (77.5)–Satt316 (103.1) | 6 (C2) | 7.4 | Sat_312 (94.1) | Tamahomare | 0.02 | 22.2 | ||
| Kyoto | Not detected | ||||||||
|
|
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| Group B saponin | Tsukuba | Not detected | |||||||
| Nagano | Satt590 (0.0)–Satt567 (20.9) | 7 (M) | 4.3 | Satt150 (3.7) | Peking | 0.05 | 5.1 | ||
| Kyoto | Not detected | ||||||||