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. 2005 Jan 20;115(2):258–267. doi: 10.1172/JCI22329

Figure 9.

Figure 9

Increased apoptosis in AtrxFoxg1Cre embryonic cortex and postnatal hippocampus. (A–I) The TUNEL assay was performed on embryos at E11.5 (A and B) and E13.5 (C and D) and at P0.5 in the hippocampus (H and I). AtrxFoxg1Cre sections (B, D, G, and I) are compared with littermate controls (A, C, F, and H). A substantial increase in the number of TUNEL-positive cells is observed in AtrxFoxg1Cre E11.5 neuroepithelial layer (compare A with B). This increase persists in the cortex of E13.5 embryos (C and D) and is not restricted to the proliferative ventricular layer. (E) Graph depicting the increased number of apoptotic cells in ATRX-deficient embryos detected by TUNEL assay. TUNEL-positive cells were counted and data expressed as a percentage of total number of cells (DAPI-positive; E11.5, n = 3, P = 0.045; E13.5, n = 4, P < 0.001). At P0.5, increased levels of TUNEL-positive cells are detected in the subventricular zone of the CA1 field (F and G) and in the developing dentate gyrus (H and I). PL, pial layer; H, hippocampus; MS, migratory stream. Missing DG in I is indicated by an asterisk. Magnification, ×20 (A, B, and F–I) and ×10 (C and D).