Figure 4.
The NLRP3 inflammasome contributes to CASP1 activation and IL1B secretion after Mn induction in BV2 cells. NLRP3 knockdown cells and nontarget control cells were treated with Mn or LPS for 6 h. (A) The protein level of NLRP3, CASP1 and cleaved IL1B were analyzed by western blot. The relative band intensities were determined by densitometry. (B) The mRNA expression of Il1b was analyzed by qPCR. (C) Levels of IL1B and IL18 in the BV2 cell culture supernatants were measured by ELISA. Statistical analysis was performed with one-way ANOVA. Data are shown as means ± SE. * P < 0.05, ** P < 0.01 when compared with the untreated group. @ P < 0.05 when compared with the NC siRNA group. # P < 0.05 when compared with the NC siRNA MnCl2 -induced group. & P < 0.05 when compared with the NC siRNA LPS-induced group. ¥ P < 0.05 when compared with the Nlrp3 siRNA Group.