Figure 8.

The activation of the NLRP3-CASP1 inflammasome induced by Mn is not restrained by controlling the autophagy maturation stage. BV2 cells were pretreated with 100 nM BAF for 24 h followed by treatment with 100 µM Mn for 6 h. (A) Representative immunocytochemistry of BV2 cells after treatment with Mn for 6 h to determine LC3B protein levels. Nuclei were stained with Hoechst. Scale bars: 20 μm. (B) The protein level of ATG5, LC3B, BECN1 and CTSB were analyzed by western blot. The relative band intensities were determined by densitometry.