Figure 2. Loss of Dicer-related helicase 1 (DRH-1) function results in enhanced susceptibility to VSV-dsRED infection.
(A) Differential interference contrast (DIC) and fluorescence micrographs (10X magnification) of N2 or drh-1 adults mock-infected or infected with 104 PFU of VSV-dsRED. Images were taken 72 hpi. Green fluorescence indicates autofluorescence obtained in the intestine. (B–F) Mean dsRED intensity measurements for N2 or drh-1 adults (n = 10/treatment) 48 hpi using the indicated VSV-dsRED dose. Measurements were taken of either the whole body (B) or of the indicated tissues (C–F). Mean dsRED measurements for the entire group (horizontal bar) and 95% confidence intervals (error bars) are shown. (G) Percentage of animals displaying a whole body dsRED signal at least two-fold above mock-infected animals by 48 hpi. (H) Percentage of animals (n = 20–30/treatment) displaying dsRED signal at the indicated times post-infection. (I) Percentage of animals from (H) alive at the indicated times post-infection. See also Figures S2–3.