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PE and EAP content is significantly decreased in SGPL1-deficient brains. Mean ± SEM of PE (n ≥ 3; unpaired Student t test, Ph3m = 0.041, Ph9m = 0.0458, Ph12m = 0.0326, Pc3m = 0.0284, Pc9m = 0.0474, Pc12m = 0.0471) and EAP (n ≥ 3; unpaired Student t test, Ph12m = 0.0277, Pc12m = 0.0828) were determined by LC/MS/MS in the hippocampus and cerebellum of control and SGPL1fl/fl/Nes mice at the indicated ages and calculated per mg of tissue.
