Figure 2.

Autophagy is altered in SGPL1-deficient brains. (A to C) Western blots and graphs showing mean ± SEM in brain extracts from control and SGPL1^fl/fl/Nes mice for: (A) BECN1 at the indicated ages (n ≥ 3; 2-way ANOVA, P_genotype = 0.0004), (B) LC3-I and LC3-II at 12 mo of age (n ≥ 3; unpaired Student t test, P_LC3 = 0.0025) and (C) SQSTM1 at 12 mo of age (n ≥ 3; unpaired Student t test, P_SQSTM1 = 0.0412). (D) Immunoblots of SQSTM1 and LC3 from hippocampi of control and SGPL1^fl/fl/Nes mice that were fed or starved (Stv) for 24 h (n ≥ 3; 2-way ANOVA, P_{SQSTM1, genotype} < 0.0001, P_{SQSTM1, stv 24 h} = 0.0011, P_{LC3, genotype} = 0.0068). Western blots of ACTB are shown as loading control. a.u., arbitrary units.