Figure 4.
Upregulation of lysosomal markers in SGPL1-deficient brains. Representative western blot images and graphs showing mean ± SEM in brain extracts from control and SGPL1fl/fl/Nes mice for: (A) LAMP2 (n ≥ 3; 2-way ANOVA, P3 m = 0.0197, P9 m = 0.013, P12 m = 0.0481) and (B) CTSD (with indication of intermediate and active variants) (n ≥ 3; 2-way ANOVA, total CTSD, Ptime = 0.0497, Pgenotype < 0.0001, and CTSD active:intermediate, P12 m = 0.0455). Western blots of ACTB are shown in all panels as loading control. a.u., arbitrary units.