Figure 5.
Accumulation of aggregate-prone proteins and deficits in spatial learning and memory in SGPL1fl/fl/Nes mice. Representative western blot images and graphs showing mean ± SEM in brain extracts of control and SGPL1fl/fl/Nes mice of the indicated ages for: (A) APP-FL (full length) and APP-CTFs (C-terminal fragments) (n ≥ 3; 2-way ANOVA, Pgenotype, APP-FL = 0.0034, Ptime, APP-CTFs = 0.0453, Pgenotype, APP-CTFs = 0.0359). (B) SNCA (n ≥ 3; 2-way ANOVA, Pgenotype = 0.0050). Western blots of ACTB are shown in all panels as loading control. (C to E) Hidden version of the Morris water maze; TQ, target quadrant with hidden platform; OQ, other quadrants. (C) Time of quadrant occupancy (2-way ANOVA, P = 0.001); (D) number of target crossings after completion of training (2-way ANOVA, P = 0.001); (E) time spent in the target area expressed as distance from the target (2-way ANOVA, P = 0.043). (F) Fear conditioning test. Shown is the relative time of activity expressed as the activity suppression ratio. Baseline activity was determined 2 min before aversive stimulus whereas time of activity was determined 1 d after associative training in a context fear-conditioning paradigm (unpaired t test, P = 0.0053). a.u., arbitrary units.