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. 2017 Feb 28;13(5):885–899. doi: 10.1080/15548627.2017.1291471

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© 2017 The Author(s). Published with license by Taylor & Francis

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 7. — PE restores autophagic flux in SGPL1-deficient neurons. (A) Representative western blot images for SQSTM1 and LC3 and graphs showing mean ± SEM in extracts from cultured neurons generated from control and SGPL1^fl/fl/Nes mice and treated or not with PE as indicated (n ≥ 3; 2-way ANOVA, P_{SQSTM1, genotype} = 0.0001, P_{SQSTM1, treatment} = 0.0158, P_{LC3, genotype} = 0.0072, P_{LC3, treatment} = 0.0293). (B) Images showing the fluorescence of the EGFP-mRFP-LC3 construct expressed in cultured neurons from control and SGPL1^fl/fl/Nes mice (2-way ANOVA, P < 0.0001). DAPI staining indicates cell nuclei in blue. Graph shows mean ± SEM of the percentage of red structures corresponding to autolysosomes with respect to the total number of structures (red and yellow) per cell (n = 20 cells in each of 2 different cultures). a.u., arbitrary units.